Products
Acetylated tubulin (Lys40) antibody
- Product Name
- Acetylated tubulin (Lys40) antibody
- Catalogue No.
- FNab00082
- Size
- 100μg
- Form
- liquid
- Purification
- Protein A+G purification
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- monoclonal
- Isotype
- IgG1
- Clone ID
- 6A10
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- tubulin, alpha 1a
- Alternative Names
- Acetylated alpha tubulin antibody, Acetylated tubulin antibody, acetylated-a-tubulin antibody, acetyl-a-tubulin antibody, alpha tubulin antibody, Alpha tubulin 3 antibody, tubulin antibody, TUBA1A antibody
- UniProt ID
- Q71U36
- Observed MW
- 52 kDa
Application
- Tested Applications
- ELISA, WB, IHC, IF
- Recommended dilution
- WB: 1:500-1:5000; IF: 1:20-1:200; IHC: 1:20-1:200
Validated Images
L02 cells were subjected to SDS PAGE followed by western blot with FNab00082(Acetylated tubulin(Lys40) Antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using FNab00082(Acetylated tubulin(Lys40) Antibody) at dilution of 1:50
- Background
- Tubulin, composed of heterodimers of alpha and beta tubulin, is the mainly component of microtubules which play important roles in cell motility, mitosis, and intracellular vesicle transport. Both alpha and beta tubulin undergo several posttranslational modifications such as polyglutamylation and acetylation/deacetylation. Tubulin acetylation occurs on lysine-40 at the N-terminal of alpha tubulin and is conserved across species. The histone deacetylase HDAC6 and SIRT2 has been identified as tubulin deacetylases. Reversible acetylation of alpha tubulin may be implicated in regulating microtubule stability, cell motility, and axon regeneration. The level of acetylated tubulin has been linked to epithelial malignancies and sensitivity to chemotherapy. In addition, acetylated tubulin has been widely used as a marker for primary cilia. This antibody is specific to the acetylated tubulin; it does not recognize non-acetylated tubulin.
